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. 2021 Mar 30;220(6):e202002084. doi: 10.1083/jcb.202002084

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© 2021 Kulkarni et al.

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Figure S4. — Nearly all AVs in dendrites and axons are positive for LAMP1. (A) Kymograph analysis of mCherry-LC3 and LAMP1-GFP in dendrites of hippocampal neurons treated with 4-AP + Bic, DMSO, or CNQX + AP5 for 30 min. Horizontal bar, 5 µm. Vertical bar, 1 min. (A’) Corresponding quantitation of the percentage colocalization between mCherry-LC3 and LAMP1-GFP in dendrites (mean ± SEM; n = 9–10 neurons from two independent experiments; 13–14 DIV). (B) Kymograph analysis of mCherry-LC3 and LAMP1-GFP in axons of hippocampal neurons treated with 4-AP + Bic, DMSO, or CNQX + AP5 for 30 min. Horizontal bar, 5 µm. Vertical bar, 1 min. (B’) Corresponding quantitation of the percentage colocalization between mCherry-LC3 and LAMP1-GFP in axons (mean ± SEM; n = 10–13 neurons from two independent experiments; 13–14 DIV).