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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Nitric Oxide. 2021 Mar 10;109-110:42–49. doi: 10.1016/j.niox.2021.03.003

Figure 4.

Figure 4.

Nitrate reductase activity of extensor digitorum longus (EDL) tissue homogenate in control conditions (ctrl), 5 days after nitrate supplementation (D0) and 7 and 21 days after high nitrate withdrawal (D7 and D21, respectively). Bars represent the amount of nitrite in nmol/g protein reduced by EDL tissue from 500 μM of exogenous nitrate during 2 hours after its addition. Oxypurinol, xanthine oxidoreductase inhibitor, was added at the same time as exogenous nitrate to the final concentration of 200μM. Gray bars represent values measured in sample without oxypurionol and green bars are values measured in the samples containing oxypurinol. EDL homogenates were kept under atmosphere of 2% oxygen, 37°C and at pH 6.5. Bars represent average of 4 rats, data are plot as average ± SD, * denotes p<0.05.