Skip to main content
. 2021 Mar 22;118(13):e2100558118. doi: 10.1073/pnas.2100558118

Fig. 3.

Fig. 3.

Subcellular location of TMEM242. (A) Analysis by SDS-PAGE of subcellular fractions (Left) from HEK293 cells expressing TMEM242 with tandem C-terminal Strep II and FLAG tags (TMEM242-t), and HeLa cells expressing TMEM242 with an N-terminal FLAG tag (TMEM242-Nt) (Right). Subcellular fractions are indicated above the panels. Proteins were detected with antibodies indicated on the left and right against FLAG and Strep II tags, the γ-subunit of ATP synthase, citrate synthase (CS), subunit NDUFS1 of complex I, histone H2A, p53 and vinculin; (B) immunofluorescence microscope images of HeLa cells expressing transiently (Upper) TMEM242-t and (Lower) TMEM242-Nt. (Upper) Two cells expressing TMEM242-t and an untransformed cell; (Lower) two cells expressing TMEM242-Nt and two untransformed cells; blue, nuclei; red, TOM20; green, TMEM242. All images were taken with the same magnification (scale bar, 20 μm).