Fig. 4.

Impact of deletion of TMEM70, TMEM242, or TMEM70 and TMEM242 together, on the composition of ATP synthase and respiratory chain complexes. (A–C) Relative abundances of subunits and the inhibitor protein IF₁ in ATP synthase immunopurified from mitoplasts of HAP1-ΔTMEM70, -ΔTMEM242, and -ΔTMEM70.ΔTMEM242 cells, respectively. IF₁-M1 is a specific mature form of IF₁ (10); f-2, is an isoform of subunit f (UniProt P56134). The median values of both relative abundance ratios for proteins derived from MS analysis in two complementary SILAC-labeling experiments are given; error bars show the range of the values. (D–F) Relative abundances of subunits of complexes I to IV (CI–CIV) and ATP synthase (CV), excluding IF₁, in extracts of mitoplasts from HAP1-ΔTMEM70, -ΔTMEM242, and -ΔTMEM70.ΔTMEM242 cells, respectively; the points represent the median of both relative abundance values of subunits in complementary experiments normalized to citrate synthase. Error bars are the mean of subunit abundances ±SD. The ratio above each complex is the number of subunits identified from CI to CV to their total subunits. Protein ratios were derived from a minimum of two peptide ratios, except for subunit c in both experiments in A, and ATP6 experiment 1 in B, where the values are derived from a single peptide ratio. In D, no ratio was obtained for subunit c. See Datasets S4–S20.