Fig. 7.

PC-mediated autophagy is involved in IOP homeostasis regulation. (A) Schematic diagram of the experimental timeline. (B) Representative immunofluorescence analysis of PC in TM tissue perfused with CH. Acetylated TUBA4A (red fluorescence) was used to identify the PC. DAPI was used to stain nuclei. Images were acquired with confocal microscope and processed by using Fiji software. (C) Profile of IOP measurement in anterior segments of porcine eye perfused with CH and subjected to double flow rate. Data are presented as mean ± SD (solid lines ± shaded regions, n = 4). (D) LC3-II protein levels in whole-tissue lysates of dissected perfused TM tissues evaluated by WB. Data are shown as the mean ± SD (n = 3). *P < 0.05 (two-tailed unpaired Student’s t test). DC, deciliated.