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. 2021 Mar 22;118(13):e2022720118. doi: 10.1073/pnas.2022720118

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Fig. 4. — UFA substrates rescue BCFA auxotrophy. (A) An 8 h subculture growth (log₁₀ CFU/mL) of ΔbmfBB::kan strain in RPMI supplemented with different 18-carbon-long FA substrates [50 μM stearic acid (18:0), 50 μM oleic acid (18:1), 50 μM linoleic acid (18:2), or 16.67 μM GTO or 0.34 mg/mL human LDL]. (B) An 8 h subculture growth (log₁₀ CFU/mL) of ΔbmfBB ΔpdhC::kan strain in RPMI only or RPMI supplemented with 50 μM 18:1 or 50 μM 18:2. (C) TLC of in vitro lipase activity assays containing 400 μM FA substrates [oleic acid (18:1) or GTO] in the presence or absence of 1 μM Geh ± 400 μM orlistat. (D) The effect of 50 μM orlistat on 8 h subculture growth (log₁₀ CFU/mL) of WT and ΔbmfBB::kan strains in RPMI supplemented with 50 μM GTO or 50 μM 18:1. (E) An 8 and 24 h subculture growth (log₁₀ CFU/mL) of the indicated ΔbmfBB::kan (ΔbmfBB) strains in RPMI supplemented with 50 μM oleic acid (18:1) or 16.67 μM GTO.