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Use of UFA substrates requires FakB2. (A) An 8 h subculture growth (log10 CFU/mL) of WT, ΔbmfBB::kan, ΔbmfBB::kan ΔfakB1, ΔbmfBB::kan ΔfakB2, and ΔbmfBB::kan ΔfakB2 + fakB2 strains in RPMI only or RPMI supplemented with 50 μM oleic acid (18:1) or 50 μM linoleic acid (18:2). (B) An 8 h subculture growth (log10 CFU/mL) of ΔbmfBB::kan and ΔbmfBB::kan ΔfakB2 strains in RPMI supplemented with BCFAP (10 mM IB, 9 mM 2MB, 9 mM IV), 0.34 mg/mL human LDL, or 16.67 μM GTO.
