FakB2 is important for bypass of BCFA requirement during S. aureus infection. (A) Bacterial burden (log10 CFU) in kidneys of mice at 96 h postinfection with 1 × 107 CFU WT (n = 12), ΔfakB1 (n = 10), and ΔfakB2 (n = 12) strains; and bacterial burden (log10 CFU) in skin abscesses of mice at 120 h postinfection with 1 × 107 CFU WT (n = 8), ΔfakB1 (n = 8), and ΔfakB2 (n = 8) strains. (B) Bacterial burden (log10 CFU) in kidneys of mice at 96 h postinfection with 1 × 107 CFU WT (n = 16), ΔbmfBB::kan (n = 18), ΔbmfBB::kan ΔfakB2 (n = 12), and ΔbmfBB::kan ΔfakB2 + fakB2 (n = 8) strains. (C) Bacterial burden (log10 CFU) in skin abscesses of mice at 120 h postinfection with 1 × 107 CFU WT (n = 8), ΔbmfBB::kan (n = 8), ΔbmfBB::kan ΔfakB2 (n = 8), and ΔbmfBB::kan ΔfakB2 + fakB2 (n = 8) strains, and representative corresponding images of skin lesions. P values were determined by a nonparametric one-way ANOVA (Kruskal–Wallis test) with Dunn’s posttest. ***P < 0.001 and ****P < 0.0001.