Figure 3. Inhibition of peritoneal macrophage efferocytosis by an anti-Mer antibody.

Apoptotic cells were prepared and co-cultured with macrophages for 4 h as described in the protocol. Anti-Mer Ab was added into the macrophage culture immediately before the co-culture with apoptotic cells. Phagocytic macrophages were then detached and stained with anti-mouse CD11b-PE antibody on ice for 30 min. Data were acquired and analyzed as in Figure 1.