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. Author manuscript; available in PMC: 2021 Apr 5.
Published in final edited form as: J Vis Exp. 2019 May 24;(147):10.3791/59731. doi: 10.3791/59731

Figure 4. RXDX-106 mediated inhibition of macrophage efferocytosis.

Figure 4.

Efferocytosis was set up as described in Figure 3. RXDX-106 was added two hrs before the co-culture with apoptotic thymocytes. Mer-deficient peritoneal macrophages were prepared similarly and served as the control group. Data were acquired and percentage of phagocytic macrophages were gated on CD11b positive cells and analyzed using the FlowJo software.