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ALT inhibits inducible STAT3 activity and STAT3 tyrosine 705 phosphorylation. PC3 cells were pretreated with a nontoxic concentration of ALT (0.01 and 0.1 μM) for 72 h. Subsequently, these cells were stimulated by IL-6 (10 ng/mL) for 30 min and analyzed with western blotting (in one repeat). GAPDH was used to normalize the expression value. ALT, Alantolactone.
