Fig. 3.

ALT decreased the expression level of stemness's factors in PC3 cells. Protein and mRNA levels of stemness's factors in PC3 cells, which were treated by nontoxic concentrations of ALT for 72 h, were evaluated with western blotting and RT-PCR. (A-E, and I) mRNA expression levels of CD133, CD44, Oct-4, Nanog, SOX2, and p53. (F-H) Protein expression levels of SOX2 and Nanog (in one repeat), GAPDH was used as a reference gene. All data represent the mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 Indicate significant differences compared to the control group. ^#P < 0.05 refers to the significant differences between indicated groups. ALT, Alantolactone; RT-PCR, real-time polymerase chain reaction.