Extended Data Figure 7. CSF1 from LepR⁺ cells is dispensable for myelopoiesis.

a. qPCR analyses showing Csf1 mRNA levels (relative to Gapdh) in Nestin-GFP^dim perivascular cells (which largely overlap with LepR⁺ perivascular cells⁴⁵). n = total 4 control mice and n = total 4 Csf1^ΔLepR mice in four experiments. b, c, Number of BM cells or the indicated populations in the femur of control or Csf1^ΔLepR mice. n = total 6 control and n = total 8 Csf1^ΔLepR mice in six experiments. d, Colony forming activity (blue: GMP, green: MDP, orange: MOP, red: GP) of the indicated progenitors FACS-purified from control (diamonds, n = 5) or Csf1^ΔLepR (circles, n = 5) total mice in four experiments. e, Number of the indicated populations in the blood of control or Csf1^ΔLepR mice. For all panels one dot equals one mouse. n = total 6 control and n= total 8 Csf1^ΔLepR mice in six experiments. Statistical differences were calculated using two-tailed Student’s T tests and p values are shown. ns = not significant.