Figure 4.

Effect of hemp peptides on apoptosis, apoptosis‐related proteins expression, and Akt/GSK3β/β‐catenin signaling in Hep3B cells. Western blots of cell lysates following treatment with 10 mg/ml hemp peptides. (a) Proapoptotic proteins Bad and cleaved caspase 3 (c‐cas 3) alongside antiapoptotic Bcl‐2. (b) p‐AKT, AKT, p‐Gsk‐3β Gsk‐3β, p‐β‐catenin, and β‐catenin protein levels in Hep3B cells. β‐actin was used as a loading control. Data are presented as the mean of three samples ± SEM (*p < .05, **p < .01, ***p < .001)