TABLE 7. Cytokine gene expression and protein production from human bronchial epitheial cells in response to a panel of PAMPs.

Cytokine gene expression levels (expressed as Fold Change compared to mock) (A) and cytokine protein levels (pg/ml) (B) produced by HBECs stimulated with agonists of PRRs in three biological triplicates as described in Figure 5.

A		Gene expression: Fold change compared to mock.
Replicate	Stimulus	IFNL1	IFNL2,3	IFNA2	IL6	IFNB	IL1B	TNF	OAS1	CCL5
1	mock	1.08776613	0.79928221	1.19999823	1.30808036	1.32516511	0.85835078	1.48185745	1.153	0.99353337
2	mock	0.80309968	1.03944458	1.1125182	1.44052637	1.8698094	1.23454434	0.97639371	0.906	1.33467285
3	mock	1.14470878	1.20364527	0.74905252	0.53069423	0.40358282	0.94368815	0.6911441	0.957	0.75412391
1	LPS	2.26403152	1.34700233	0.57470547	8.53626546	1.8206117	1.87712863	1.83645884	0.823	1.0446748
2	LPS	0.88769411	1.19076621	0.68154988	5.79591359	1.22705776	1.84912206	2.02753574	0.631	1.07399805
3	LPS	0.08570655	0.79481745	0.45996989	6.10265885	0.35862051	1.897624	1.23594805	1.199	1.24693359
1	R848	0.12626965	1.00125676	0.51241564	1.55489994	0.70863548	1.45388658	1.32152016	1.572	1.1366199
2	R848	1.19116929	0.85538217	0.66924748	0.85064115	0.6127652	1.99729347	1.76992468	0.922	1.12812089
3	R848	0.12662991	1.68360147	1.15521676	1.01779155	1.43743636	1.99409404	2.74516011	0.774	0.99089092
1	CpG	0.70965977	1.09906755	1.33329012	0.46222685	0.50048681	1.20562076	0.9860895	0.84	0.39827961
2	CpG	0.13897165	1.34321804	0.69414175	0.6819143	0.79420964	1.43290523	1.14569699	0.812	0.57587246
3	CpG	0.12914228	1.11462397	0.64504556	1.42512396	0.93157229	1.42900761	1.48854516	0.621	1.43524691
1	poly(I:C)	152.57392	269.767186	0.4036708	26.6623935	11.3580198	1.93664808	17.2269121	74.773	707.642877
2	poly(I:C)	108.38442	180.054819	0.30374745	17.2593057	5.68820494	1.8594426	10.8601403	68.343	606.266728
3	poly(I:C)	85.9993752	190.486567	0.47608847	15.0663454	6.22247983	1.66321366	11.4573603	59.053	447.126246
1	3pRNA	540.068187	3029.78274	1.86181381	6.10027114	194.395249	1.49218139	1.88036466	137.506	45.9807249
2	3pRNA	517.943584	2788.98084	1.52979042	7.93482956	220.443617	1.60169859	1.51268518	84.662	26.2228929
3	3pRNA	492.066973	3308.17133	4.74806775	12.8623593	250.691648	1.59525226	1.44130584	82.562	29.1500038
1	c-GAMP	5.53607866	7.18213991	0.48317833	2.39282148	3.11833951	1.9264935	2.53645673	3.688	4.11139724
2	c-GAMP	7.39465192	20.6455335	1.49077696	2.09857544	2.6244464	1.5801579	1.60226108	2.784	2.43542714
3	c-GAMP	7.72934409	16.7636075	1.13941914	0.94170136	2.76246264	1.50864361	2.75171454	5.355	4.62987816
B		Protein leves (pg/ml)
Replicate	Stimulus	CXCL10	IFN-α2	IFN-λ1	IFN-λ2,3	IFN-β	IFN-γ	IL-1β	IL-6	IL-8
1	mock	15.285	0.669	6.761	22.01	5.93	0.97	6.47	16.61	4788.965
2	mock	29.836	1.182	11.246	48.61	19.131	1.7	14.5	29.59	8570.515
3	mock	41.735	1.31	13.626	52.74	18.155	2.1	15.45	36.05	11628.465
1	LPS	14.706	0.937	6.761	22.95	5.931	0.97	5.41	13.1	4280.483
2	LPS	20.446	0.862	8.663	26.73	6.212	0.97	10.08	19.67	6299.808
3	LPS	23.085	0.981	9.112	43.53	17.831	1.56	9.32	24.3	6747.016
1	R848	18.828	0.894	9.553	35.17	10.894	1.32	13.06	27.03	6533.723
2	R848	19.263	0.894	8.663	32.37	9.462	1.22	12.33	23.85	7074.38
3	R848	18.62	0.729	8.205	22.01	7.629	0.97	9.07	21.63	5521.605
1	CpG	11.033	0.574	6.188	13.41	5.931	0.97	4.61	5.9	4872.379
2	CpG	13.423	0.81	8.205	23.9	5.931	0.97	8.17	9.58	6586.405
3	CpG	13.784	0.831	9.112	29.55	6.492	1.51	9.07	14.13	6489.428
1	poly(I:C)	8008.032	1.159	324.505	63.66	261.164	1.41	16.15	456.82	19457.155
2	poly(I:C)	9717.693	1.925	370.669	90.86	302.521	2.61	20.79	693.4	23680.885
3	poly(I:C)	9967.855	2.144	436.984	114.72	336.636	2.82	24.12	762.95	25097.665
1	3pRNA	7364.434	1.047	140.25	61.85	241.609	1.41	18.45	48.65	9523.413
2	3pRNA	7845.313	1.047	159.882	68.18	236.71	1.51	21.23	53.93	12032.822
3	3pRNA	8424.087	1.345	167.729	77.14	264.936	1.51	19.8	61.12	11463.586
1	c-GAMP	133.378	1.024	16.947	44.92	28.268	1.36	10.96	64.01	5924.002
2	c-GAMP	102.066	0.64	10.832	22.95	5.931	0.97	8.29	54.51	5697.244
3	c-GAMP	115.437	1.069	12.456	36.1	16.54	1.41	13.3	70.44	6955.899