Skip to main content
. Author manuscript; available in PMC: 2022 Apr 2.
Published in final edited form as: J Mol Biol. 2021 Feb 4;433(7):166846. doi: 10.1016/j.jmb.2021.166846

Figure 2.

Figure 2.

POT1-TPP1 binds and destabilizes 6ThT22 G4s. (A) Stoichiometric binding EMSAs of POT1-TPP1 with 6ThT22 in K+ buffer. (B) Experimental (black) and modeled (orange) sensorgrams to describe the interactions of immobilized 6ThT22 with POT1-TPP1 proteins in K+ buffer. (C) Time-dependent changes in ellipticity of 6ThT22 with or without POT1-TPP1. (D) Plot showing changes in CD amplitude (295 nm) of 6ThT22 DNA as a function of increasing molar ratio of POT1-TPP1 protein:DNA. (E) Kinetic model for protein binding and G4 unfolding of 6ThT22 in K+. (F) Correlation between EMSA experimental data and fitted data using kinetic model.