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. 2021 Mar 8;6(5):e141962. doi: 10.1172/jci.insight.141962

Figure 1. Characterization of EVs released from 3T3-L1 adipocytes and human subcutaneous adipose tissue.

Figure 1

Analysis of size distribution, showing the size and number of EVs released from 3T3-L1 adipocytes (Ad-EVs) (A) and human subcutaneous adipose tissue (SAT-EVs) (B) using the NanoSight technology (n = 3). Insets show representative micrographs obtained by TEM of purified Ad-EVs and SAT-EVs (scale bar: 100 nm). (C and D) Representative Western blot images for EV markers Alix, CD9, and CD63 in 3T3-L1 adipocytes and Ad-EVs (C) and in SAT and SAT-EVs (D) (n = 3). (E and F) Representative Western blot for adiponectin, leptin, FABP4, and PPARγ in 3T3-L1 cells and Ad-EVs (E) and in SAT and SAT-EVs (F). Actin served as internal control (n = 3). (G and H) Representative gene expression for adiponectin, leptin, FABP4, PPARγ1, and PPARγ2 in 3T3-L1 and Ad-EVs (G) and in SAT and SAT-EVs (H), as assessed by RT-PCR. 18s rRNA served as internal control (n = 3).