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. 2021 Mar 8;6(5):e145185. doi: 10.1172/jci.insight.145185

Figure 3. Effect of PM2.5 on AHR in both human primary keratinocytes and organotypic skin.

Figure 3

Expressions of AHR (red) in both cultured HEKs (A and B) and organotypic skin (C and D) were evaluated using immunofluorescence staining and demonstrated a reduction in FLG expression after PM2.5 exposure. Wheat germ agglutinin–conjugated FITC (green) was used to stain the cytoskeleton. Nuclei were visualized with DAPI (blue). Data are representative of 3 independent experimental repetitions. The data are shown as the mean ± SEM. n = 3 per group. Scale bar: 50 μm. **P < 0.01 by 2-tailed Student’s t test.