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Gene expression of FLG was examined using real-time RT-PCR in cultured HEKs stimulated with PM2.5 or combinations of each inhibitor and PM2.5. (A) NF-κB inhibitor. (B) ERK1/2 inhibitor. (C) P38 inhibitor. (D) SP600125 inhibitor. Data are representative of 3 independent experimental repetitions. The data are shown as the mean ± SEM. n = 3 per group. **P < 0.01 by 1-way ANOVA with Tukey-Kramer post hoc test.
