Skip to main content
. 2021 Mar 8;6(5):e141245. doi: 10.1172/jci.insight.141245

Figure 6. Foxp3+ Tregs from αTIGIT-treated memory mice exhibit deteriorated differentiation and activation.

Figure 6

Splenocytes were harvested at 48 hours after CLP from memory and previously naive septic mice treated with αTIGIT or isotype Ab. (A) Summary data of the percentage of Foxp3+ Tregs in the 4 groups. (B) Absolute numbers of Foxp3+ Tregs among 4 groups. n = 8–9/group. Results were representative of 2 independent experiments. Groups were compared using 1-way ANOVA analysis and Tukey’s multiple comparison test. (C) Representative flow plots depicting Helios expression on CD4+Foxp3+ T cells. (D) Summary data of the percentage of Helios-expressing cells among Foxp3+ Tregs. (E) Summary figure depicting CTLA-4 MFI of Foxp3+ Tregs. (F and G) Summary data of the percentage of CD69+ and CD62L+ cells among Foxp3+ Tregs. (H) Splenocytes from memory septic mice were harvested at 48 hours and restimulated with PMA/ionomycin ex vivo for 4 hours and analyzed for IL-10. Results represent 2 independent experiments. Data are shown as the mean ± SEM. Groups were compared using 1-way ANOVA analysis and Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.