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. 2021 Mar 8;6(5):e145217. doi: 10.1172/jci.insight.145217

Figure 5. Anti–cit-TNC antibodies do not cross-react with each other.

Figure 5

Sera that were double-reactive with peptides cit-TNC17 or cit-TNC56 and cit-TNC56, cit-TNC17, or cit-TNC5 were diluted 1:100, incubated with 1, 10, and 100 μg/mL of peptides for 2 hours and centrifuged at 10,000g for 10 minutes, and the supernatant then added to peptide-coated plates for analysis by ELISA. (A) No cross-reactivity between anti–cit-TNC17 and both anti–cit-TNC56 and anti–cit-TNC5. (B) Limited cross-reactivity between anti–cit-TNC56 and both anti–cit-TNC17 and anti–cit-TNC5. Each panel was run on a separate plate.