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. 2021 Mar 8;6(5):e145662. doi: 10.1172/jci.insight.145662

Figure 7. UMCD6 induces upregulation of NKG2D on NK and CD8+ T cells.

Figure 7

(A) NK-92 cells were incubated with 10 μg/mL of IgG or UMCD6 and harvested after 4 hours. Real-time PCR revealed significantly higher levels of mRNA for the activating receptor NKG2D, as well as perforin and granzyme B upon incubation with UMCD6, while mRNA for the inhibitory receptor NKG2A was downregulated. Data are expressed as mean ± SD; *P < 0.05. (B and C) Representative flow cytometry plots showing NKG2D-positive NK and CD8+ T cells. PBMCs from 6 donors (n = 6) were isolated as described in Methods and subsequently cultured with 10 μg/mL IgG or UMCD6. Increase of cell surface expression of NKG2D was evident 72 hours after treatment with UMCD6 on NK cells (gated on CD3–CD56+ cells) (B) and CD8+ T cells (gated on CD3+CD8+ cells) (C).