Table 3. Protein identities, in decreasing abundance, in immunodominant bands 1W and 2W in Western blots with EE as antigen.
Proteins were identified by LC-MS/MS of corresponding areas in SDS-PAGE gels, 1G and 2G.
| Accession number | Annotation | MW (kDa) | Peptides* (95%) |
|---|---|---|---|
| Area 1G (≈ 150–200 kDa) | |||
| A0A077ZE83 | Vitellogenin N and VWD and DUF1943 domain containing protein | 198.527 | 241 |
| A0A077Z8E4 | Heat shock protein 70 | 130.299 | 3 |
| Area 2G (≈ 37–45 kDa) | |||
| A0A077Z5Q5 | Poly-cysteine and histidine tailed protein isoform 2 | 50.940 | 66 |
| A0A077YX57 | Enolase | 49.513 | 18 |
| A0A077ZHV3 | Glyceraldehyde-3-phosphate dehydrogenase | 37.536 | 15 |
| A0A077ZE37 | Actin | 41.838 | 14 |
| A0A077Z3K7 | Phosphoglycerate kinase | 44.724 | 12 |
| A0A077Z8X2 | Dolichyl- diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit | 48.787 | 12 |
| A0A077ZF21 | Adenosylhomocysteinase | 47.827 | 10 |
| A0A077Z3H9 | Tubulointerstitial nephritis antigen | 50.458 | 9 |
| A0A077YXR0 | Calponin domain containing protein | 40.694 | 7 |
| A0A077ZJA3 | 3 ketoacyl coenzyme A thiolase | 43.402 | 6 |
| A0A077YYL7 | Elongation factor 1-alpha | 51.086 | 6 |
| A0A077Z1Z4 | Serpin domain containing protein | 43.328 | 6 |
*The number of distinct peptides having at least 95% confidence.