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. 2021 Apr 2;54(5):e9700. doi: 10.1590/1414-431X20209700

Table 2. The overexpression/silencing of lentiviral vector and sequences.

No. STEM
Silencing of lentiviral vector GV280 hU6-MCS-Ubiquitin-EGFP-IRES-puromycin
Insertion sequence of silencing lentiviral vector virus hsa-miR-183-5p-inhibition(18159-1) TATGGCACTGGTAGAATTCACT
Insertion of silencing lentiviral vector negative control virus CON137 TTCTCCGAACGTGTCACGT
Overexpression of lentiviral vector GV369 Ubi-MCS-SV40-EGFP-IRES-puromycin
Insertion sequence of overexpression lentiviral vector virus hsa-mir-183(17525-1) CCAAGGGAGTGGGCAGGCTAGGAGCAGGGAACGGGCATCGTGGGCCGCTGGTCTCTCCGCAGGGTCGGCAGGCCGCAGAGTGTGACTCCTGTTCTGTGTATGGCACTGGTAGAATTCACTGTGAACAGTCTCAGTCAGTGAATTACCGAAGGGCCATAAACAGAGCAGAGACAGATCCACGAGGGCCTCCGGAGCACCTTACCCACTTCTGCCTTGAGTGCTCCTAGACGTCGGAAACAGGCTGCTTCCAAGGGTGCAGGGA
Insertion sequence of overexpression lentiviral vector negative control virus CON238 NA