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. 2021 Mar 22;10:e61276. doi: 10.7554/eLife.61276

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© 2021, Everetts et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — (A-C) Max projections over all images slices (epithelium + AMPs) from wing discs with FLAG tag inserted at the N-terminus of Ths (Bl. 77476). Single image slice of the disc proper (DP) notum (D-F) or underlying AMPs (G-I), corresponding to the region within the yellow dashed box in A. (J-L) Close up of AMPs within the dashed yellow box in G. In all images, FLAG is visualized with anti-FLAG antibody (red), and AMPs are visualized with anti-Cut antibody (cyan). Anti-Cut antibody imperfectly stains AMPs, due to the use of saponin to optimize the visualization of anti-FLAG staining. Note the puncta stained by anti-FLAG antibody within the AMPs. Microscopy scale bars = 100 μm in A-I, and 10 μm in J-L.