Figure 1.

PCR process diagram of primers with incomplete homology with the template. Schematic diagram of the reaction assumed by PCR from partially matched primers. DNA elongation may start from primers on which partially 3′-end matches (B). On the end of second cycle, the 3′-end of the elongated DNA completely match with a primer (C). On the end of third cycle, synthesized DNA are completely matched on both ends of synthesized DNA (D).