Table 1.
Primer sets for the main experiment.
| Primer no. | Primer name | Sequence |
|---|---|---|
| 1 | aim_1f | GTCCAGGGCTTCACACATGCTA |
| aim_22r | TGTTACCAACTTTCATGACGTG | |
| 2 | aim_71f | AGCGCAACCCTCACCTTATGTT |
| aim_94r | GGGACCGGATTTTTGAGATTAG | |
| 3 | aim_122f | TTCAGTTGGGCACTCGTAAGGA |
| aim_94r | GGGACCGGATTTTTGAGATTAG | |
| 4 | aim_94f | CTAATCTCAAAAATCCGGTCCC |
| aim_143r | CCTTCACGAGTTTCACCTTAGT | |
| 5 | aim_94f | CTAATCTCAAAAATCCGGTCCC |
| aim_147r | CTTCACCCCCTTCACGAGTTTC | |
| 6 | aim_161f | GAGGTGGAGCGAATCCCAGAAA |
| aim_194r | CTTACCAAGCATACCTTAGGCA | |
| 7 | aim_263f | CAAATCCCAGAAAGCCGCTCTC |
| aim_250r | ACCAGCCCTGCCGTCGGCGCCT | |
| 8 | aim_348f | GTGTTGCCTAGCAATAGGATCT |
| aim_282r | TGCTGCCCTCTGTCTATGCCAT | |
| 9 | aim_386f | GCTGAGGACTCTAATTGAACTG |
| aim_394r | AGACAGCTTTTAAGGGATTTCC | |
| 10 | aim_386f | GCTGAGGACTCTAATTGAACTG |
| aim_461r | CCGATCCGGACTGAGACAGCTT | |
| 11 | aim_394f | GGAAATCCCTTAAAAGCTGTCT |
| aim_436r | CGAGCGTCTTTGGGTACTCCTG | |
| 12 | aim_468f | GGCGGAGGAAATCCTAAAAACT |
| aim_515r | CTTCAGATACTTCGGGTGCGAC | |
| 13 | aim_555f | ACGGGACTGCCCGCGAAAGCGG |
| aim_562r | GGGCCCACCTTTTTGCGATTAG | |
| 14 | aim_599f | GTGCTACAACGGGTAGCGAAAC |
| aim_652r | CCGCCGAGGCGGAGTTGGGTCA | |
| 15 | aim_764f | CTTATCCTTTGTTGCCAGCGGT |
| aim_744r | CGACATACTTTATGAGGTCCGC | |
| 16 | aim_812f | CTGCCAGTGATAAACTGGAGGA |
| aim_744r | CGACATACTTTATGAGGTCCGC | |
| 17 | aim_842f | TCTCATAAAACCGTTCTCAGTT |
| aim_848r | TGTTACAAACTCTCGTGGTGTG | |
| 18 | aim_1016f | CTAATCGGAAAAAGCCGGCCTC |
| aim_1036r | ATGAATTACACCTTGGGCGGCT | |
| 19 | aim_1209f | GTGTCGGTAGTTACAGGTGTCT |
| aim_1159r | ATTGTCGTGGCCATTGTAGCGT | |
| 20 | aim_1248f | CGCCGTGACCGGCGGAGGAAGG |
| aim_1193r | CCGCGCCATGGCTGATACGCGG | |
| 21 | aim_1175f | TCGCCTAAACGTGGTCTCAGTG |
| aim_1264r | TCCCAGTCGCGGCCCCTGCCCT | |
| 22 | aim_1177f | GCCTAAACGTGGTCTCAGTGCA |
| aim_1264r | TCCCAGTCGCGGCCCCTGCCCT | |
| 23 | aim_1316f | CTAGTGGGACAGCCGGAGTAAT |
| aim_1285r | TGCAATCCGAACTAAGACAAGG | |
| 24 | aim_1332f | CCGGAGTAATCCGGAGGAAGGT |
| aim_1276r | AGGTTTTTGAGGTTGGCTCACT | |
| 25 | aim_1285f | CCTTGTCTTAGTTCGGATTGCA |
| aim_1293r | GCTTCTGGCAAAACCGACTTTC | |
| 26 | aim_1401f | TGAGGTGTCGGCTTAAGTGCCA |
| aim_1368r | GCTAGCTGCCTTCTGTACCCCC | |
| 27 | aim_1401f | TGAGGTGTCGGCTTAAGTGCCA |
| aim_1383r | TTTGGGATTAGCATACGGTCAC | |
| 28 | aim_1415f | GAGGTGTCGGCTTAAGTGCCAT |
| aim_1368r | GCTAGCTGCCTTCTGTACCCCC | |
| 29 | aim_1447f | AGGTCATGCTGAGGACTCTGGA |
| aim_1368r | GCTAGCTGCCTTCTGTACCCCC | |
| 30 | aim_1447f | AGGTCATGCTGAGGACTCTGGA |
| aim_1391r | TTCGATCCGAACTGAGAGAGGA | |
| 31 | aim_1391f | TCCTCTCTCAGTTCGGATCGAA |
| aim_1465r | CCCTAGGACGATCCTTGCGGTT | |
| 32 | aim_1549f | GGGTAATGCCGGGTACTCACAG |
| aim_1504r | CATTGTCCCTGCCACTGTAGCG | |
| 33 | aim_1504f | CGCTACAGTGGCAGGGACAATG |
| aim_1550r | TAGCTCGGGGACTTCCGATGAA | |
| 34 | aim_1758f | GCCAATACAAACAGTTGCAAAT |
| aim_1775r | TACCAGCTCTCATAGTTTGACG | |
| 35 | aim_1770f | CTGTAAAGTTGGTCTCAGTTCG |
| aim_1775r | TACCAGCTCTCATAGTTTGACG | |
| 36 | aim_1770f | CTGTAAAGTTGGTCTCAGTTCG |
| aim_1811r | CTACCCTAGACATGCGCTTCCT | |
| 37 | aim_1948f | CAAAGGGCAGCGACATAGTGAT |
| aim_1984r | ATGAGCCGTAGCTGATGCCCAT | |
| 38 | aim_2085f | AGTACAGAAGGTAGCAAGATCG |
| aim_2138r | AACGTATTCACGGCGTTATGGC | |
| 39 | aim_2109f | GATGGAGCAAATCCTTAAAGCT |
| aim_2164r | TCAACGACTTAAGGTAAAACCA | |
| 40 | aim_2292f | GGTTAAGTCCCCTAACGAGCGA |
| aim_2247r | ATGACTTTGCAGCCTAGCAACG | |
| 41 | aim_2547f | TCGAGTACATGAAGTTGGAATC |
| aim_2581r | TACGGTTAGGCCTGCTACTTCA | |
| 42 | ai2_1242_f | TACTTTGTCTAACGAGACTGCC |
| ai2_1242_r | CGAACTGAGACCAACTTTACAG | |
| 43 | ai2_100_f | ACGAGCCGGAGGAAGGAGG |
| ai2_100_r | ACCCCGGGAACGTATTCACC | |
| 44 | ai2_1213_f | CCTAAACCCTGTCGTGGTGCAG |
| ai2_1213_r | TAGCTCGGGGACTTCCGATGAA | |
| 45 | ai2_1325_f | TAAGGGGACTGCCCCGGATAAC |
| ai2_1325_r | GCGCTTTCTGAGATTCGCTCAG | |
| 46 | ai2_6_f | CAAGTCGAGCGGAGAAGATTT |
| ai2_6_r | GGTATTACCCATCCTTTCGGAT | |
| 47 | ai2_1194_f | GCGGGTGACCGTATGCTAATCC |
| ai2_1194_r | CTTGCGGTTACGTACTTCAGGT | |
| 48 | ai2_1315_f | CGTTGCTAGGCTGCAAAGTCAT |
| ai2_1315_r | GCGGCTCCGGCGACTTCGGATG | |
| 49 | ai2_1147_f | CGCCGTGACCGGCGGAGGAAGG |
| ai2_1147_r | CACTGAGACCACGTTTAGGCGA | |
| 50 | ai2_23_f | GAGACTGCCGGTGACAAACC |
| ai2_23_r | AGTTGCAGACTCCAATCCGGA | |
| 51 | ai2_1244_f | GCCAATACAAACAGTTGCAAAT |
| ai2_1244_r | TACCAGCTCTCATAGTTTGACG | |
| 52 | ai2_1125_f | ACCGCTGCAACCCCGCGAGGGT |
| ai2_1125_r | TGGGCGGCTGCTCCCTTGCGGT | |
| 53 | ai2_1238_f | GGCACAGGTGGTGCACGGCCGT |
| ai2_1238_r | GGCATAAGGGGCACGAGTACCT | |
| 54 | ai2_1166_f | CCGGAGTAATCCGGAGGAAGGT |
| ai2_1166_r | TGCAATCCGAACTAAGACAAGG | |
| 55 | ai2_1143_f | TGCCGCCGTGACCGGCGGAGGA |
| ai2_1143_r | CACTGAGACCACGTTTAGGCGA | |
| 56 | ai2_1124_f | ACCGCTGCAACCCCGCGAGGGT |
| ai2_1124_r | AGCGCACCGACTTCTAGTGCAA | |
| 57 | ai2_1284_f | ACGAGACTGCCTGGGTTAACCA |
| ai2_1284_r | AGCTTTAAGGATTTGCTCCATC | |
| 58 | ai2_1288_f | CTGCCTGGGTTAACCAGGAGGA |
| ai2_1288_r | GAACTGGGGCCAGCTTTAAGGA | |
| 59 | ai2_1090_f | AAAGGAGACTGCCAGTGATAAA |
| ai2_1090_r | TCCAATCCGGACTACGACATAC | |
| 60 | ai2_1142_f | GTGTCGGTAGTTACAGGTGTCT |
| ai2_1142_r | CAACTCCGCCTTCACGGGGGCG | |
| 61 | ai2_1195_f | GCGGGTGACCGTATGCTAATCC |
| ai2_1195_r | CCCTAGGACGATCCTTGCGGTT | |
| 62 | ai2_101_f | GTCGTCGTCAGCTCGTGCC |
| ai2_101_r | CTCCTTCCTCCGCCTCGTC | |
| 63 | ai2_1189_f | CGTCGTAAGATGTGAGGAAGGT |
| ai2_1189_r | TTCGATCCGAACTGAGAGAGGA | |
| 64 | ai2_1088_f | CTTATCCTTTGTTGCCAGCGGT |
| ai2_1088_r | TCCAATCCGGACTACGACATAC | |
| 65 | ai2_1192_f | GGGGGTACAGAAGGCAGCTAGC |
| ai2_1192_r | CTTGCGGTTACGTACTTCAGGT | |
| 66 | ai2_1303_f | GCCATAACGCCGTGAATACGTT |
| ai2_1303_r | CTTCATCCTAGTCATCAGCCTC | |
| 67 | ai2_1102_f | AAGTTGGGCAGTCTAAGGTGAC |
| ai2_1102_r | TCTTGCAGCTCTTTGTACCGTC | |
| 68 | ai2_54_f | CGGGTGAGTAACACGTATCTAA |
| ai2_54_r | TCTCAGTTCGGCTACGTATCAT | |
| 69 | ai2_1275_f | TGATATGGAGCGAATCCCCAAA |
| ai2_1275_r | GTCTGCCTCCTGCAAGCAGGTT | |
| 70 | ai2_1327_f | CTGAGCGAATCTCAGAAAGCGC |
| ai2_1327_r | TTGCCTGGGTTGGGCCACCGGC | |
| 71 | ai2_10_f | CTGGCGGCGTGGATAAGACA |
| ai2_10_r | ATGGGCTATTCCCCACTTCAG | |
| 72 | ai2_1071_f | AGCGATGCCACCCGGCAACGGG |
| ai2_1071_r | CCTGCCCGTAGGCTCCCGGCGA |
Primer pair number, primer name and base sequence (5′ → 3′) used in the experiments for RNN-training are shown. Primers with the same primer pair number are used as a set of primers.