Table 2.
Primer sets for the test.
| Primer set no. | Primer name | Primer sequence |
|---|---|---|
| 1 | 1_f_180 | AACGCGCTGCGAGCCTGTGA |
| 1_r_369 | CCCACAAGGGTTAGGCCACT | |
| 2 | 1_f_180 | AACGCGCTGCGAGCCTGTGA |
| 1_r_397 | ATCGCCGATCCCACCTTCGA | |
| 3 | 1_f_180 | AACGCGCTGCGAGCCTGTGA |
| 1_r_400 | CCAATCGCCGATCCCACCTT | |
| 4 | 1_f_180 | AACGCGCTGCGAGCCTGTGA |
| 1_r_408 | ACTTCGTCCCAATCGCCGAT | |
| 5 | 1_f_186 | CTGCGAGCCTGTGAGGGTGA |
| 1_r_369 | CCCACAAGGGTTAGGCCACT | |
| 6 | 1_f_186 | CTGCGAGCCTGTGAGGGTGA |
| 1_r_397 | ATCGCCGATCCCACCTTCGA | |
| 7 | 1_f_186 | CTGCGAGCCTGTGAGGGTGA |
| 1_r_400 | CCAATCGCCGATCCCACCTT | |
| 8 | 1_f_186 | CTGCGAGCCTGTGAGGGTGA |
| 1_r_408 | ACTTCGTCCCAATCGCCGAT | |
| 9 | 2_f_50 | TCAGTTGGGCACTCGTAAGG |
| 2_r_342 | TGGCAAAGACCACTTCGGGT | |
| 10 | 4_f_251 | CTAAAGCCACCCCCAGTTCA |
| 4_r_395 | CTCTTCGCCTGACTTCGGGT | |
| 11 | 4_f_251 | CTAAAGCCACCCCCAGTTCA |
| 4_r_403 | TCGGCAGGCTCTTCGCCTGA | |
| 12 | 5_f_0 | TGCCTGGGAGCCCTAGCACA |
| 5_r_228 | CCCCTTACGGGTTCGCTTCC | |
| 13 | 5_f_223 | CAGAGGGAAGCGAACCCGTA |
| 5_r_410 | TCCGGGGGTTGGGATAGCGA | |
| 14 | 6_f_66 | GCCTAGCAATAGGATCTCTC |
| 6_r_211 | GGGCATAGTTTAGGGATTGG | |
| 15 | 6_f_211 | CCAATCCCTAAACTATGCCC |
| 6_r_363 | AGACGACCTGAGCACTTCTG | |
| 16 | 6_f_211 | CCAATCCCTAAACTATGCCC |
| 6_r_385 | TACTAATCACAACTTAGGGC | |
| 17 | 7_f_205 | AATCCCTTAAAAGCTGTCTC |
| 7_r_347 | AGCGTCTTTGGGTACTCCTG | |
| 18 | 8_f_78 | ACTGCCCAGATCAACTGGGA |
| 8_r_349 | TGGCTTCAGATACTTCGGGT | |
| 19 | 8_f_78 | ACTGCCCAGATCAACTGGGA |
| 8_r_359 | TCCTTGCGGTTGGCTTCAGA | |
| 20 | 9_f_87 | GACTGCCCGCGAAAGCGGGA |
| 9_r_181 | GTTGCCGGGTGGCATCGCTT | |
| 21 | 10_f_226 | TCCCTAAAAAGCATCCTCAG |
| 10_r_381 | AGGCGGAGTTGGGTCACTGA | |
| 22 | 12_f_190 | GGCATATACAAAGAGAAGCG |
| 12_r_395 | TAAGCGCCCTCCCGAAGGTT | |
| 23 | 12_f_216 | CGAGAGCAAGCGGACCTCAT |
| 12_r_395 | TAAGCGCCCTCCCGAAGGTT | |
| 24 | 12_f_236 | AAAGTATGTCGTAGTCCGGA |
| 12_r_395 | TAAGCGCCCTCCCGAAGGTT | |
| 25 | 13_f_203 | GGTACAAAGAGCTGCAAGAC |
| 13_r_397 | CTCCAAAAAGGTTACCCCAC | |
| 26 | 14_f_64 | GCAAGGGGGCCCTCTGGAGA |
| 14_r_341 | TAGAGCACTCCCTTCTCCCA | |
| 27 | 15_f_60 | TGGCGAAACCGCCTCGGATA |
| 15_r_349 | CTCCCTTGCGGTTAGCGCAC | |
| 28 | 18_f_17 | TGTCGGTAGTTACAGGTGTC |
| 18_r_177 | GATCTGCACTGAGACCACGT | |
| 29 | 18_f_173 | CTAAACGTGGTCTCAGTGCA |
| 18_r_330 | TCCCCGACTGGGGTTAGCAC | |
| 30 | 19_f_83 | TAGTGGGACAGCCGGAGTAA |
| 19_r_177 | AGGTCGCATCCCGTTGTCCT | |
| 31 | 19_f_90 | ACAGCCGGAGTAATCCGGAG |
| 19_r_371 | CTATCCGAAGATTCGGTCAC | |
| 32 | 19_f_196 | TCGCGAGAGTGAGCCAACCT |
| 19_r_346 | TCTGGCAAAACCGACTTTCG | |
| 33 | 19_f_196 | TCGCGAGAGTGAGCCAACCT |
| 19_r_371 | CTATCCGAAGATTCGGTCAC | |
| 34 | 20_f_225 | ATCCCAAAATCCTCTCTCAG |
| 20_r_382 | GACGATCCTTGCGGTTACGT | |
| 35 | 21_f_61 | GGGTAATGCCGGGTACTCAC |
| 21_r_211 | ACCACGACAGGGTTTAGGGG | |
| 36 | 21_f_61 | GGGTAATGCCGGGTACTCAC |
| 21_r_217 | ATCTGCACCACGACAGGGTT | |
| 37 | 21_f_61 | GGGTAATGCCGGGTACTCAC |
| 21_r_227 | GCAACCCTCAATCTGCACCA | |
| 38 | 21_f_179 | AATGGGCTGCAACGCCGTAA |
| 21_r_359 | GTTAGCTCGGGGACTTCCGA | |
| 39 | 21_f_216 | AAACCCTGTCGTGGTGCAGA |
| 21_r_348 | GACTTCCGATGAACCCGACT | |
| 40 | 23_f_151 | ATGACGTCAGGTACTCGTGC |
| 23_r_437 | CCCCCCTCACCAGGTTCTCC | |
| 41 | 24_f_84 | CTGCCAACGTAAGTTGGAGG |
| 24_r_360 | CTTGCGGTTAGCAACACGGT | |
| 42 | 26_f_74 | AGACTGCCCGTGTTAAGCGG |
| 26_r_169 | TCACTATGTCGCTGCCCTTT | |
| 43 | 26_f_74 | AGACTGCCCGTGTTAAGCGG |
| 26_r_358 | CTGCAAGCAGGTTGGCGCAA | |
| 44 | 28_f_165 | AATGGGGCGGACAGAGCGTT |
| 28_r_333 | CCCCCGCTTTGGTGGCTTGA | |
| 45 | 28_f_165 | AATGGGGCGGACAGAGCGTT |
| 28_r_397 | ACTTAGTCCCCATCACGGGT | |
| 46 | 28_f_170 | GGCGGACAGAGCGTTGCTAG |
| 28_r_339 | GGATGCCCCCCGCTTTGGTG | |
| 47 | 28_f_173 | GGACAGAGCGTTGCTAGGCT |
| 28_r_333 | CCCCCGCTTTGGTGGCTTGA | |
| 48 | 28_f_192 | TGCAAAGTCATGCTAATCGC |
| 28_r_333 | CCCCCGCTTTGGTGGCTTGA | |
| 49 | 28_f_192 | TGCAAAGTCATGCTAATCGC |
| 28_r_397 | ACTTAGTCCCCATCACGGGT | |
| 50 | 28_f_204 | CTAATCGCAAAAACCGTTCC |
| 28_r_397 | ACTTAGTCCCCATCACGGGT | |
| 51 | 29_f_237 | GCGAATCTCAGAAAGCGCTC |
| 29_r_437 | CCCAGTCGCCAGCCATACCA | |
| 52 | 30_f_128 | CAATGCTACGGACAAAGGGC |
| 30_r_306 | TTCGGGCGTGGCCAACTTCC | |
| 53 | 30_f_128 | CAATGCTACGGACAAAGGGC |
| 30_r_328 | CCACAAGGGTTGGAGTAACG | |
| 54 | 30_f_128 | CAATGCTACGGACAAAGGGC |
| 30_r_342 | TTCGGCGTCCTCCTCCACAA |
Primer pair number, primer name and base sequence (5′- > 3′) used in the experiments for RNN-test are shown. Primers with the same primer pair number are used as a set of primers.