Table 3.
Base pair (primer base–template base) | Primer-template | Primer hairpin or dimer | |||
---|---|---|---|---|---|
Initial stage | Middle stage | ||||
Forward | Reverse | Forward | Reverse | ||
A-T, T-A | a | f | p | u | k |
C-G, G-C | b | g | q | v | l |
A-A, A-G, G-A, G-G, C-C | c | h | r | w | m |
T-T, T-C, C-T | d | i | s | x | n |
C-A, A-C, G-T, T-G | e | j | t | y | o |
A symbol for generating pseudo-words for RNN learning. The codes are set in the nucleotide duplex on each base pair at the complementary position. Mismatched base pairs such as A-A, T-T and C-A may appear within the partially complementary region. Base-pairs are grouped based on influence for stability of partially complementary strands.