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. 2021 Mar 23;14:613887. doi: 10.3389/fnmol.2021.613887

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Copyright © 2021 Yang, Zhang, Chen, Zhu, Li, Ouyang, Chu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of expression levels of miR-184 in brain on the injured brain following ischemia stroke. Rats were subjected to middle cerebral artery occlusion (MCAO) operation to induce ischemic stroke model with or without intracerebroventricular injection of miR-184 adenovirus (Ad-miR-184). After 24 h following MCAO, a cohort of rats was sacrificed, and brain tissue was taken for TTC staining and qRT-PCR to evaluate the infarct volume and miR-184 expression in brain. Other rats were subjected to analysis of neurological function deficit 1, 3, 7, 14, 21, and 28 day after MCAO operation using mNSS system. (A) mNSS scores in various groups (n = 8–9). (B) TTC-stained brain slice images 24 following ischemia-reperfusion (n = 6 in each group). (C) Quantification analysis of the infarct volume. (D) Relative expression of miR-184 in the brain (n = 6 in each group). Values are mean ± SD. *p < 0.05 vs. the sham or vehicle.