Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 23;14:613887. doi: 10.3389/fnmol.2021.613887

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Yang, Zhang, Chen, Zhu, Li, Ouyang, Chu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

MiR-184 target gene and verification. MiR-184 target gene was predicted using starBase v.2.0 miRNA database (starBase.sysu.edu.cn/), and was verified using dual-luciferase reporter assays. Transduction of SH-SY5Y cells with hsa-PPAP2B interference lentivirus (PPAP2B Inf V) or empty vector and attack with oxygen-glucose deprivation/reoxygenation (OGD/R). Apoptosis was analyzed by flow cytometry to evaluate the effect of the miR-184 target gene on neuronal apoptosis. (A) MiR-184 target is phosphatidic acid phosphatase genes type 2B (PPAP2B) mRNA, which can base-pair with miR-184 at its 3’-untranslated region sequences. (B) Luciferase activity. (C) Flow cytometry results of cell apoptosis. (D) Percent of cell apoptosis. Experiments were repeated in triplicate. Values are mean ± SD. *p < 0.05 vs. the control or vehicle.