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. 2021 Mar 23;15:631501. doi: 10.3389/fnins.2021.631501

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Copyright © 2021 Xu, Yang, Huang and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Selection of suitable concentration and time for 6-OHDA treatment of cells. The cell viability of SH-SY5Y cells treated with different concentrations (10, 50, 100, 150, and 200 μM) of 6-OHDA at 0, 3, 6, 12, 24, and 48 h was detected using MTT assay. Each experiment was repeated for three times independently. Data are presented as mean ± standard deviation. One-way ANOVA was employed for the comparisons among multiple groups and Dunnett’s multiple comparisons test was applied for the post hoc test, ^∗∗p < 0.01 vs. control group at the same time point.