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. 2021 Mar 23;15:631501. doi: 10.3389/fnins.2021.631501

FIGURE 2.

FIGURE 2

AS-IV improved SH-SY5Y cell viability and repaired morphological damage induced by 6-OHDA. (A) Effect of different concentrations of AS-IV (25, 50, 100, 150, and 200 μM) on the SH-SY5Y cell viability were detected using MTT assay. (B) Protective effect of AS-IV (25, 50, and 100 μM) on SH-SY5Y cells treated with 6-OHDA (100 μM) was observed using MTT assay. (C) The expression of MAP2 was detected using immunofluorescence staining. (D) Effect of AS-IV (25, 50, and 100 μM) on morphology of SH-SY5Y cells treated with 6-OHDA (100 μM) was detected using Hoechst 33258 staining; the arrowhead indicated the different situations of cell morphological damage, including the reduction and roundness of cell morphology, and the dense fluorescent granules. Each experiment was repeated for three times independently. Data are presented as mean ± standard deviation. One-way ANOVA was employed for the comparisons among multiple groups and Tukey’s multiple comparison test was applied for the post hoc test. **p < 0.01 vs. control group; ##p < 0.01 vs. 6-OHDA groups.