FIGURE 3.

AS-IV inhibited the levels of inflammatory factors and oxidative stress in 6-OHDA-treated SH-SY5Y cells. SH-SY5Y cells were treated with different concentrations of AS-IV (25, 50, and 100 μM) for 2 h, and then treated with 6-OHDA (100 μM) for 24 h. (A–C) Levels of IL-1β, IL-6, and TNF-α in 6-OHDA-treated SH-SY5Y cells were detected using the ELISA kits. (D–F) Changes of MDA, ROS, and SOD level in 6-OHDA-treated SH-SY5Y cells were detected using corresponding kits. Each experiment was repeated for three times independently. Data are presented as mean ± standard deviation. One-way ANOVA was employed for the comparisons among multiple groups and Tukey’s multiple comparison test was applied for the post hoc test, ^∗p < 0.05, ^∗∗p < 0.01 vs. control group; ^#p < 0.05, ^##p < 0.01 vs. 6-OHDA groups.