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. 2021 Mar 23;12:649547. doi: 10.3389/fphys.2021.649547

FIGURE 1.

FIGURE 1

Hydrogen peroxide and hypoxia resulted in DNA damage, G2/M arrest and senescence in HK-2 cells. (A) Representative western blots analysis of γH2A.X (ser139), and bar graphs showed the fold changes compared to control group at different time points. (B) Flow cytometry analysis of cell cycle. *P < 0.05, ****P < 0.0001, compared with control group. (C) SA-β-gal staining analysis at different time points. (D) EdU incorporation analysis at 48 h. (E) Representative SA-β-gal staining at 48 h, Scale bars, 50 μm. (F) Representative EdU incorporation images at 48 h showed decreased proliferation rate and larger cell size in H2O2 and hypoxia groups, Scale bars, 50 μm. n = 3–5/group. Data are means ± SD. SA-β-gal, Senescence Associated β-Galactosidase; EdU, 5-Ethynyl-2′-deoxyuridine.