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. 2021 Mar 23;12:649547. doi: 10.3389/fphys.2021.649547

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Copyright © 2021 Jia, Kang, Tan, Ren, Qu, Tang, Liu, Wang, Xiong and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nicotinamide mononucleotide (NMN) administration before ischemia and during acute phase attenuated uIRI induced interstitial fibrosis at day 21 after surgery. (A,B) Representative sirius red staining images and semiquantitative analysis of the percentage of positive sirius red staining area. Scale bars, 100 μm. (C,D) Representative images of alpha-smooth muscle actin (α-SMA) immunofluorescence staining in cortex and outer medulla and semiquantitative analysis of percentage of positive area. Scale bars, 100 μm. (E) Representative western blots and semiquantitative analysis of α-SMA. (F) Representative western blots and semiquantitative analysis of Collagen IV (Col IV). n = 6–7/group. Data are means ± SD.