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. 2021 Mar 23;12:649462. doi: 10.3389/fphys.2021.649462

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Copyright © 2021 Abd el-Rady, Dahpy, Ahmed, Elgamal, Hadiya, Ahmed, Sayed, Abdeltawab, Abdelmohsen, Farrag, Ashmawy, Khairallah and Galal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs of immunohistochemical staining of e-NOS in fundic mucosa showing: (A) the control group showing highly positive strong expression of e-NOS in glandular epithelial cells and in blood capillaries in the lamina propria (curved arrow). (B) IND-treated group showing weak positive expression confined mainly in blood capillaries of the lamina propria (curved arrows). (C) PLN-treated group showing moderate positive expression in the vascular endothelium, surface epithelium and glandular epithelial cells (curved arrow). (D) PPE-treated group showing weak positive expression more prominently in the vascular endothelium of the lamina propria (curved arrow) (Immunostaining for eNOS × 200, scale bar = 50 μm). (E) quantitative analysis of the mean count of e-NOS immunopositive cells in the fundic mucosa of the studied groups. Data are presented as mean ± SD. Kruskal-Wallis H test followed by the Mann-Whitney U tests were used for analyzing the results. ^asignificant as compared to the control group (p ≤ 0.05). ^bsignificant as compared to IND-treated group (p ≤ 0.05), ^csignificant as compared to PPE-treated group (p ≤ 0.05).