Figure 10.

Effects of changes in iron availability in HeLa cells or RAW264.7 macrophages on intracellular STM. HeLa cells (A, B) or RAW264.7 macrophages (C, D) were infected at MOI 5 with STM WT, ΔssaV, or ΔsifA strains as indicated, each containing the iron reporter p5008. At 1 h p.i., Fe(NO₃)₃ (orange) or 2.2-dipyridyl (green) were added to final concentrations of 100 µM to infected HeLa cells (A, B) or RAW264.7 macrophages (C, D) and maintained for the rest of the infection period. Host cells were lysed 16 h p.i., released STM were fixed, and subjected to FC to quantify sfGFP intensities of P_sitA-positive STM. Data for STM WT [p5008] in HeLa cells (A) or RAW264.7 macrophages (C) of a representative experiment are shown. Representative quantification of X-mean sfGFP intensities for STM WT, ΔssaV, or ΔsifA in HeLa cells (B) or RAW264.7 macrophages (D) at 16 h p.i. Mean values and standard deviations of P_sitA-positive bacterial populations from triplicates of a representative experiment from three biological replicates are shown. Statistical analyses are indicated as for Figure 3 .