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. 2021 Mar 23;12:651060. doi: 10.3389/fimmu.2021.651060

Figure 2.

Figure 2

Cytokine production by Spl-stimulated T cells. T cells were isolated from whole blood of CF patients (n = 5) and healthy volunteers (n = 5) and stimulated with a mixture of recombinant SplA, SplB, SplD, SplE and SplF (5 µg/mL each) in the presence of CD14+ antigen-presenting cells. Supernatants were taken at day 9 and cytokine concentrations measured by a cytometric bead array. Concentrations were normalized to 1 million T cells. Production of Th2 cytokines (IL 4, IL 5, IL 13) and IL 6 was significantly higher in CF patients compared to healthy controls, IL 10 release in tendency lower, while there were no significant differences in the concentrations of IFNγ, IL 17A, or IL 17F. Medians (grey bars) with interquartile ranges are indicated; *P < 0.05; **P < 0.01; Mann Whitney U test. CF, cystic fibrosis.