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. 2021 Mar 23;12:641300. doi: 10.3389/fpls.2021.641300

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Copyright © 2021 Zheng, Xu, Wang, Tang, Wei, Ji, Wang and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Premature senescence identification of mutant leaves. (A–F) Ultrastructural analysis of chloroplasts in wild type and mutants by transmission electron microscopic. C, chloroplast; CE, chloroplast membrane; CW, cell wall; OG, osmiophilic plastoglobuli. (G) Determination of photosynthetic pigment content. (H) Expression assay of chloroplast synthesis-related genes. (I) Expression assay of photosynthetic-related genes. (J) Expression assay of senescence-related genes. Error bars represent the standard deviations of three biological replicates. * and **, significant differences at P < 0.05 and P < 0.01, respectively (Student’s t-test).