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. 2021 Mar 5;24(4):102273. doi: 10.1016/j.isci.2021.102273

Figure 4.

Figure 4

Cisplatin-induced DNA damage does not result in subcellular mis-localization of DNMT1

(A) Confocal images demonstrating the results of anti-DNMT1 immunofluorescence. Cells treated with 0 uM, 5 uM, and 10 uM cisplatin were stained with DAPI nuclear stain (blue) and anti-DNMT1 antibody (red). The outline of the cell membrane can be seen with the addition of the brightfield lens in the right-hand panels. DNMT1 appears nuclear in cells treated with different cisplatin concentrations. Scale bar, 5 um.

(B) Confocal images demonstrating the results of anti-5mC immunofluorescence on cells treated with either 0 uM, 5 uM, or 10 uM cisplatin. Cells were stained with DAPI nuclear stain (blue) and anti-5mC antibody (yellow). Similar levels of 5mC fluorescence are observed in cells treated with different cisplatin concentrations. Scale bar, 50 um.