Figure 2.

DNA-binding proteins stay closely associated with the nucleoid during the target search

(A–C) Localizations of Pol1 (PolA-PAmCherry), (B) RNAP (RpoC-PAmCherry), and (C) ribosomal protein S1 (S1-PAmCherry) molecules relative to the nucleoid. Left to right: transmitted light image (scale bar, 1 μm), SytoGreen-stained nucleoid DNA with a segmented cell outline, and maps of mobile (blue) and immobile (red) molecule tracks. Histograms show localizations and SytoGreen fluorescence profiles projected onto the long cell axis (green line).

(D) Average spatial distributions of Pol1, RNAP, and ribosomal protein S1 immobile and mobile molecules.

See also Figure S1.