REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial strains
MG1655 F- lambda- ilvG- rfb-50 rph-1	Coli Genetic Stock Centre	CGSC#: 7740
TB28 I-SceICS-ilvA	Bernhardt and de Boer, 2005	N/A
TB28 I-SceICS-ilvA-FRT (3953 kb)	This study	N/A
TB28 I-SceICS-ydeO	This study	N/A
TB28 I-SceICS-ydeO-FRT-cat-FRT (1580 kb)	This study	N/A
TB28 I-SceICS-ilvA-FRT, I-SceICS-ydeO-FRT (this strain is referred to as OT hereafter)	This study	N/A
RNAP-PAmCherry (MG1655 rpoC-PAmCherry-FRT-kan-FRT)	Stracy et al., 2015	N/A
HU-PAmCherry (MG1655 hupB-PAmCherry-FRT-kan-FRT)	Stracy et al., 2015	N/A
HN-S-PAmCherry (MG1655 Hns-PAmCherry-FRT-kan-FRT)	Stracy et al., 2015	N/A
FIS-PAmCherry (MG1655 fis-PAmCherry-FRT-kan-FRT)	Uphoff et al., 2013	N/A
LacI-PAmCherry (MG1655 LacI-PAmCherry)	Garza de Leon et al., 2017	N/A
Pol1-PAmCherry (MG1655 polA-PAmCherry-FRT-kan-FRT)	Uphoff et al., 2013	N/A
LigA-PAmCherry (MG1655 ligA-PAmCherry-FRT-kan-FRT)	Uphoff et al., 2013	N/A
UvrA-PAmCherry MG1655 uvrA-PAmCherry-FRT-kan-FRT	Stracy et al., 2016	N/A
MutS-PAmCherry (MG1655 mutS-PAmCherry-FRT-kan-FRT)	Uphoff et al., 2016	N/A
TopoIV-PAmCherry (MG1655 parC-PAmCherry-FRT-kan-FRT)	Zawadzki et al., 2015	N/A
MukB-PAmCherry (MG1655 mukB-PAmCherry-FRT-kan-FRT)	Badrinarayanan et al., 2012	N/A
GyrA-PAmCherry (MG1655 gyrA-PAmCherry-FRT-kan-FRT)	Stracy et al., 2019	N/A
recA- strain (TB28 recAT233C-Tet)	Lesterlin et al., 2014	N/A
MinC-Ypet (minC-Ypet)	Bisicchia et al., 2013	N/A
OT RNAP-PAmCherry (OT rpoC-PAmCherry-FRT-kan-FRT)	This study	N/A
OT Pol1-PAmCherry (OT polA-PAmCherry-FRT-kan-FRT)	This study	N/A
OT LigA-PAmCherry (OT ligA-PAmCherry-FRT-kan-FRT)	This study	N/A
OT MukB-PAmCherry (OT mukB-PAmCherry-FRT-kan-FRT)	This study	N/A
OT LacI-PAmCherry (OT / p lacI-PAmCherry)	This study	N/A
OT LacI41-PAmCherry (OT / p lacI41-PAmCherry)	This study	N/A
OT Free PAmCherry (OT pBAD\HisB PAmCherry1)	This study	N/A
OT FIS-PAmCherry (OT fis-PAmCherry-FRT-kan-FRT)	This study	N/A
OT recA- (OT recAT233C-Tet)	This study	N/A
OT RNAP PAmCherry recA- (OT rpoC-PAmCherry-FRT-kan-FRT recAT233C-Tet)	This study	N/A
OT Pol1-PAmCherry recA- (OT polA-PAmCherry-FRT-kan-FRT recAT233C-Tet)	This study	N/A
OT LigA-PAmCherry recA- (OT ligA-PAmCherry-FRT-kan-FRT recAT233C-Tet)	This study	N/A
OT MukB-PAmCherry recA- (OT mukB-PAmCherry-FRT-kan-FRT recAT233C-Tet)	This study	N/A
OT LacI-PAmCherry recA- (OT / P lacI-PAmCherry recAT233C-Tet)	This study	N/A
OT LacI41-PAmCherry recA- 9OT / P lacI41-PAmCherry recAT233C-Tet)	This study	N/A
OT Free PA-mCherry recA- (OT pBAD\HisB PAmCherry1 recAT233C-Tet)	This study	N/A
Chemicals, peptides, and recombinant proteins
EZ Rich Defined Medium RDM (Teknova Inc)	VWR	200059-658
Deposited data
Raw and analyzed data	This study	http://data.mendeley.com/login?redirectPath=/datasets/4skb3txv92/draft?a=11f7a88d-70c3-43e0-9883-b1d313bc6c92
Software and algorithms
MATLAB	MathWorks	https://uk.mathworks.com/products/MATLAB.html
NIS-Elements AR	Nikon	https://www.microscope.healthcare.nikon.com
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
MicrobJ	Ducret et al., 2016	https://www.microbej.com
MicrobeTracker	Sliusarenko et al., 2011	http://microbetracker.org/