Fig. 7.

Removal of hepatic Scap normalized Mboat7-induced steatosis (n = 5–7 per group). A: mRNA levels of Mboat7, Scap, Acc1, and Scd1 in WT (black), Mboat7 LSKO (red), Scap LSKO (blue), and Mboat7/Scap dKO (green) mice. Total RNA from each mouse liver was subjected to real-time PCR. Cyclophilin was used as the invariant control. Values represent the amount of mRNA relative to that in the WT mice, which are arbitrarily assigned a value of 1. B: Hepatic TGs in all four mouse lines. C: Hepatic total cholesterol in all four mouse lines. Data are presented as mean ± SD. The experiment was repeated with similar results. Mboat7 LSKO, Mboat7 liver-specific KO mice; TG, triglyceride.