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. 2021 Apr 6;12:226. doi: 10.1186/s13287-021-02257-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Evaluation of blood vessel formation. a Early angiogenesis in the wound beds was visualized on day 3 postwounding by hematoxylin and eosin staining. Staining showed an increased number of blood vessels forming in the wound in the ADSC+PRP group compared with the other groups. The black arrows indicate blood vessels. Scale bars = 50 μm. b Representative images of tissue sections from diabetic rats immunostained for CD31 (green), CD34 (pink), and α-SMA (red) and quantification of c the capillary density and the proportion of CD31+, CD34+, and α-SMA+ cells 7 and 14 days postwounding (× 200, scale bars = 50 μm; × 400, scale bars = 20 μm; *p < 0.05; n = 3 wounds per group)