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A size-exclusion (HiPrep Sephacryl 26/60 S-200 HR column) chromatogram of purified vimentin coil 1B. The column was run in the gel filtration buffer, as specified in the Materials and Methods, at 2 mL/min. The 29.8 kDa mark on the x-axis serving as a calibration standard corresponds to the dimer of the DNA binding domain of FLI1 protein (with the dimer molecular weight of 29.8 kDa) [48].
