Table 1.
Potentiated versus un-potentiated channel openings for various types of AChRs in the presence of ACh and NS9283
| Receptor type (number of patches) | [ACh] μM | [NS9283] μM | Total openings (n) | Proportion of openings | SE | |
|---|---|---|---|---|---|---|
| Un-potentiated | Potentiated | |||||
| (α4)3(β2)2 (n = 3) | 10 | 10 | 4895 | 0.42 | 0.58 | ± 0.01 |
| (α4)3(β2)2 (n = 6) | 1 | 30 | 2214 | 0.39 | 0.61 | ± 0.04 |
| (α4)3(β2)2 (n = 4) | 10 | 30 | 3462 | 0.22 | 0.78 | ± 0.06 |
| (α4)3(β2)2 (n = 6) | 50 | 30 | 27,025 | 0.26 | 0.74 | ± 0.03 |
| (α4)3(β2)2 (n = 4) | 100 | 30 | 1885 | 0.45 | 0.55 | ± 0.02 |
| (α4)3(β2W176A)2 (n = 6) | 50 | 30 | 1752 | 0.90 | 0.10 | ± 0.01 |
| β2α4β2α4α4 (n = 6) | 50 | 30 | 2776 | 0.19 | 0.81 | ± 0.04 |
| β2W176Aα4β2α4α4 (n = 6) | 50 | 30 | 1403 | 0.84 | 0.16 | ± 0.01 |
| β2α4β2W176Aα4α4 (n = 8) | 50 | 30 | 810 | 0.71 | 0.29 | ± 0.04 |
| β2W176Aα4β2W176Aα4α4 (n = 10) | 50 | 30 | 473 | 0.86 | 0.14 | ± 0.05 |
| β2α4β2α4H142Vα4 (n = 8) | 50 | 30 | 2080 | 0.45 | 0.55 | ± 0.09 |
Averaged proportions of potentiated versus un-potentiated openings are given for the indicated number of patches (n) along with ± the standard error (SE). For each experimental condition, the total number of openings from n patches is indicated. The table summarizes results displayed in Figs. 1, 2, 3, and 4