Table 2.
Sequence of primers used for qPCR
| Genes | Forward | Reverse |
|---|---|---|
| β-ACTIN | GGCCGCACCACTGGCATTGTCAT | AGGTCCAGACGCAGGATGGCG |
| FSHR | TCACAGTCCCTCGGTTCCTT | AGCATCACAGCCTGCTCCA |
| LHCGR | ATGGGGCTCTACCTGCTACTCA | GAGCCACCCTCCAAGCATAA |
| GDF9 | CTCTGCCTCTTCCTCCTCCACTG | GGTGAATGAGTACGGTGCTCTTGG |
| BMP15 | ACCATGCCATCATTCAGAGCCTTG | CGTTGGTCTCAATCAGGAGGATGC |
| CYP11A1 | GGCTCCAGAGGCCATAAAGA | ACTCAAAGGCGAAGCGAAAC |
| STAR | GACTTTGTGAGTGTCGGCTGTA | ATCCCTTGAGGTCAATGCTG |
| 3β-HSD | CACTGACCTGGGCTGATGAC | GTGGCGAGAAGCAGACAAGA |
| CYP17A1 | TCCAAGCCAAGACGAAC | TTTACCACAGAGGCAGAAG |
Abbreviations: FSHR follicle-stimulating hormone receptor, LHCGR luteinizing hormone/chorionic gonadotropin receptor, GDF9 growth differentiation factor 9, BMP15 bone morphogenetic protein 15, CYP11A1 cytochrome P450 family 11 subfamily A member 1, STAR steroidogenic acute regulatory protein, 3βHSD 3 β-hydroxysteroid dehydrogenase, CYP17A1 cytochrome P450 family 17 subfamily A member 1