Fig. 3.

The influence of ALPL mutation on cell proliferation and apoptosis of DPSCs. a, b The ALP activities (a) and the ALPL expression (b) on protein were examined in DPSCs from normal and patient with HPP. c The effective CFU of DPSCs in culture medium was compared between control and HPP groups in vitro. d, e The cell cycle (d) and cell apoptosis (e) of DPSCs from normal and HPP patient were performed by flow cytometry analysis. The data were analyzed by Student’s t test and presented as the mean ± s.d. of triplicate samples from a representative experiment. *p < 0.05, **p < 0.01, ***p < 0.001