Fig. 5.

LiCl restored odontoblastic differentiation of HPP DPSCs by promoting canonical Wnt pathway activation. a Canonical Wnt pathway-related key proteins, including total and phosphorylation of GSK3β, and total and active β-catenin in DPSCs from health and HPP groups were examined by Western blotting. b Immunofluorescence staining of p-GSK3β and active β-catenin were performed in the first molars of ALPL^+/+ and ALPL^+/− mice. Scale bar = 100 μm. c–e. The expression of total and phosphorylation of GSK3β, and total and active β-catenin in DPSCs from health and health with pLko.1-ALPL shRNA (c), DPSCs from HPP and HPP with pLenti-ALPL (d), or DPSCs from HPP and HPP treated with LiCl (e). f Alizarin Red staining was performed in DPSCs from HPP and HPP treated with LiCl after cultured in odontoblastic medium for 3 weeks. g The protein expression of DMP1 and DSPP were examined in DPSCs from HPP and HPP treated with LiCl after cultured in odontoblastic medium for 7 days. The data were presented as the mean ± s.d. of triplicate samples and analyzed by Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001